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iwp4  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc iwp4
    A Schematic illustration of the treatment scheme in iPSCs prior to short-read-NGS RNAseq analysis at day 2 (d2). Timepoint of 2 hours pulse-treatment with 18 nM doxorubicin (Dox) is indicated by a lightning symbol. B Gene concept network of GO-term enrichments and associated downregulated genes of pairwise comparison between Dox-treated iPSCs and the respective control. Number of genes, logFC-value, and GO-terms are listed in the respective legend. C Scheme of iPSC differentiation into iPSC-derived cardiomyocytes (iCMs) following a chemically defined protocol. At defined days (d), cardiac basal media (CBM) was supplemented without B-27 minus insulin (-INS) or with insulin (+INS). In addition, growth factors such as CHIR99021 (CBM-INS1) or <t>IWP4</t> (CBM-INS2) were added at specific days of the differentiation protocol. Days with negative numbers represent the time of culturing iPSCs until initiation of cardiac differentiation. On day 7 differentiation of iPSCs into iPSC-derived cardiomyocytes (iCMs) is completed. Representative images of iCMs acquired with Leica DM IL LED Fluo Cellfactory (Leica). Days (d) of cardiac differentiation are indicated. Magnification: 20x, scale bar: 300 µm. D Determination of sensitivity of iCMs against Dox. iCMs were pulse-treated for 2 hours with increasing doses of doxorubicin (Dox) and viability was determined using an MTT assay. The mean ± SEM of three independent biological replicates (n=3; N=4-8) is shown.
    Iwp4, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iwp4/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    iwp4 - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Preconditioning of human iPSCs with doxorubicin causes genome-wide transcriptional reprogramming in iPSC-derived cardiomyocytes linked to mitochondrial dysfunction and impaired cardiac regeneration"

    Article Title: Preconditioning of human iPSCs with doxorubicin causes genome-wide transcriptional reprogramming in iPSC-derived cardiomyocytes linked to mitochondrial dysfunction and impaired cardiac regeneration

    Journal: bioRxiv

    doi: 10.1101/2025.04.18.649376

    A Schematic illustration of the treatment scheme in iPSCs prior to short-read-NGS RNAseq analysis at day 2 (d2). Timepoint of 2 hours pulse-treatment with 18 nM doxorubicin (Dox) is indicated by a lightning symbol. B Gene concept network of GO-term enrichments and associated downregulated genes of pairwise comparison between Dox-treated iPSCs and the respective control. Number of genes, logFC-value, and GO-terms are listed in the respective legend. C Scheme of iPSC differentiation into iPSC-derived cardiomyocytes (iCMs) following a chemically defined protocol. At defined days (d), cardiac basal media (CBM) was supplemented without B-27 minus insulin (-INS) or with insulin (+INS). In addition, growth factors such as CHIR99021 (CBM-INS1) or IWP4 (CBM-INS2) were added at specific days of the differentiation protocol. Days with negative numbers represent the time of culturing iPSCs until initiation of cardiac differentiation. On day 7 differentiation of iPSCs into iPSC-derived cardiomyocytes (iCMs) is completed. Representative images of iCMs acquired with Leica DM IL LED Fluo Cellfactory (Leica). Days (d) of cardiac differentiation are indicated. Magnification: 20x, scale bar: 300 µm. D Determination of sensitivity of iCMs against Dox. iCMs were pulse-treated for 2 hours with increasing doses of doxorubicin (Dox) and viability was determined using an MTT assay. The mean ± SEM of three independent biological replicates (n=3; N=4-8) is shown.
    Figure Legend Snippet: A Schematic illustration of the treatment scheme in iPSCs prior to short-read-NGS RNAseq analysis at day 2 (d2). Timepoint of 2 hours pulse-treatment with 18 nM doxorubicin (Dox) is indicated by a lightning symbol. B Gene concept network of GO-term enrichments and associated downregulated genes of pairwise comparison between Dox-treated iPSCs and the respective control. Number of genes, logFC-value, and GO-terms are listed in the respective legend. C Scheme of iPSC differentiation into iPSC-derived cardiomyocytes (iCMs) following a chemically defined protocol. At defined days (d), cardiac basal media (CBM) was supplemented without B-27 minus insulin (-INS) or with insulin (+INS). In addition, growth factors such as CHIR99021 (CBM-INS1) or IWP4 (CBM-INS2) were added at specific days of the differentiation protocol. Days with negative numbers represent the time of culturing iPSCs until initiation of cardiac differentiation. On day 7 differentiation of iPSCs into iPSC-derived cardiomyocytes (iCMs) is completed. Representative images of iCMs acquired with Leica DM IL LED Fluo Cellfactory (Leica). Days (d) of cardiac differentiation are indicated. Magnification: 20x, scale bar: 300 µm. D Determination of sensitivity of iCMs against Dox. iCMs were pulse-treated for 2 hours with increasing doses of doxorubicin (Dox) and viability was determined using an MTT assay. The mean ± SEM of three independent biological replicates (n=3; N=4-8) is shown.

    Techniques Used: Comparison, Control, Derivative Assay, MTT Assay



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    Image Search Results


    A Schematic illustration of the treatment scheme in iPSCs prior to short-read-NGS RNAseq analysis at day 2 (d2). Timepoint of 2 hours pulse-treatment with 18 nM doxorubicin (Dox) is indicated by a lightning symbol. B Gene concept network of GO-term enrichments and associated downregulated genes of pairwise comparison between Dox-treated iPSCs and the respective control. Number of genes, logFC-value, and GO-terms are listed in the respective legend. C Scheme of iPSC differentiation into iPSC-derived cardiomyocytes (iCMs) following a chemically defined protocol. At defined days (d), cardiac basal media (CBM) was supplemented without B-27 minus insulin (-INS) or with insulin (+INS). In addition, growth factors such as CHIR99021 (CBM-INS1) or IWP4 (CBM-INS2) were added at specific days of the differentiation protocol. Days with negative numbers represent the time of culturing iPSCs until initiation of cardiac differentiation. On day 7 differentiation of iPSCs into iPSC-derived cardiomyocytes (iCMs) is completed. Representative images of iCMs acquired with Leica DM IL LED Fluo Cellfactory (Leica). Days (d) of cardiac differentiation are indicated. Magnification: 20x, scale bar: 300 µm. D Determination of sensitivity of iCMs against Dox. iCMs were pulse-treated for 2 hours with increasing doses of doxorubicin (Dox) and viability was determined using an MTT assay. The mean ± SEM of three independent biological replicates (n=3; N=4-8) is shown.

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    doi: 10.1101/2025.04.18.649376

    Figure Lengend Snippet: A Schematic illustration of the treatment scheme in iPSCs prior to short-read-NGS RNAseq analysis at day 2 (d2). Timepoint of 2 hours pulse-treatment with 18 nM doxorubicin (Dox) is indicated by a lightning symbol. B Gene concept network of GO-term enrichments and associated downregulated genes of pairwise comparison between Dox-treated iPSCs and the respective control. Number of genes, logFC-value, and GO-terms are listed in the respective legend. C Scheme of iPSC differentiation into iPSC-derived cardiomyocytes (iCMs) following a chemically defined protocol. At defined days (d), cardiac basal media (CBM) was supplemented without B-27 minus insulin (-INS) or with insulin (+INS). In addition, growth factors such as CHIR99021 (CBM-INS1) or IWP4 (CBM-INS2) were added at specific days of the differentiation protocol. Days with negative numbers represent the time of culturing iPSCs until initiation of cardiac differentiation. On day 7 differentiation of iPSCs into iPSC-derived cardiomyocytes (iCMs) is completed. Representative images of iCMs acquired with Leica DM IL LED Fluo Cellfactory (Leica). Days (d) of cardiac differentiation are indicated. Magnification: 20x, scale bar: 300 µm. D Determination of sensitivity of iCMs against Dox. iCMs were pulse-treated for 2 hours with increasing doses of doxorubicin (Dox) and viability was determined using an MTT assay. The mean ± SEM of three independent biological replicates (n=3; N=4-8) is shown.

    Article Snippet: From day 4 to day 5, 1x B-27 supplement without insulin (ThermoFisher), 50 μg / mL ascorbic acid (Sigma-Aldrich) and 5 μM IWP4 (STEMCELL Technologies Inc.) was added to CBM.

    Techniques: Comparison, Control, Derivative Assay, MTT Assay